ICP 22 (1975)

  • second generation of ICP flow cytometry instrumentation
  • version with 1 optical parameter:   ICP 21
  • version with 2 optical parameters:  ICP 22
  • version with 3 optical parameters:  ICP 23
  • licensed to Phywe AG, Göttingen (Germany)

Technical Data

Flow Chamber

_ sheath flow principle
_ 200µm orifice diameter
_ suspension rate max. 8ml/min
_ rinsing liquid consumption max. 40ml/min
_ max. cell concentration: 30000/ml
_ max. sample rate 60000 cells/min

Optics

_ Light Source: high pressure mercury lamp 100W
   (HBO 100W OSRAM) with separate highly stabilized
   lamp current supply
_ Köhler Illumination
_ Excitation filter: selectable according to
   application, for example BG12 + (BG38 + KG1)
_ Reflector and barrier filter: selectable according to 
   application, for example for 2 parameters FT580 +
   interference filter 510
_ Objective: Zeiss Neofluar 63/1, 25 oil
_ Photomultiplier: exchangable; standard model
   green-red sensitive (S 20 RCA, C 7164 R)
_ High Voltage 0-1000 V, continuously adjustable

Fluidic System

_ Low noise vacuum piston pump, driven by a
   Ferraris motor
_ Tubing: 3 electrically checked valves for rinsing
   liquid, drainage, and drainage system ventilation

Indicator
Electronics

_ oscilloscope for signal observation,
   2 parameters per screen
_ 2 LED arrays to indicate average illumination and
   lamp alignment

Dimensions

_ Detector Instrument (Desktop Unit):
   400 x 430 x 480 mm (LxWxH), Weight: 25 kg
_ Electronic Cabinet:
   600 x 600 x 1500 mm (LxWxH), Weight: 50kg
_ Stabilized Lamp Current Supply:
   215 x 330 x 230 mm (LxWxH), Weight: 11.5 kg

Power Supply

_ alternating current 220 V 50 Hz
_ current requireddetector system 0.35 A
_ electronic cabinet 0.70 A
_ adaption transformer 110/220 V AC, 50-60 Hz